possibility of hav diagnosis in milk by elisa technique
نویسندگان
چکیده
food borne viral infections are recognized increasingly as human illneses. hepatitis a virus (hav) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk . in this study for approaching to best diagnosis method of hav in milk by elisa technique, various dilutions of hav antigen (1 ml/l, 10-3 ml/l, 10-6 ml/l, 10-9 ml/l) were added to uht milk . various treatments for virus(antigen) isolation, showed that milk with acidic coagulation, following filtration with paper and membrane filters, had the most optical density. it`s indicated that this treatment resulted the best diagnosis . also it has been revealed that this method has not any sensitivity for quantitative determination of hav(antigen) in milk.
منابع مشابه
Determination of Aflatoxin M1 in Milk by ELISA Technique in Mashad (Northeast of Iran)
The aim of this study was to detect the amount of aflatoxin M1 (AFM1) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 97.6% of the examined milk samples ...
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عنوان ژورنال:
پژوهش های علوم و صنایع غذایی ایرانجلد ۱، شماره ۱، صفحات ۰-۰
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